Identifying viral parameters from in vitro cell cultures

Identifying viral parameters from in vitro cell cultures

Current in vitro cell culture studies of viral replication deliver detailed time courses of several virological variables, like the amount of virions and the number of target cells, measured over several days of the experiment. Each of these time points solely provides a snap-shot of the virus infection kinetics and is brought about by the complex interplay of target cell infection, and viral p...

Activation in vitro of rheumatoid synovial collagenase from cell cultures.

Rheumatoid synovial cells dissociated from matrix and adherent to culture dishes released a latent form of collagenase into culture medium. Previous studies have shown that the latent enzyme does not complex with alpha2-macroglobulin and binds to fibrillar substrate. We now show that serum-free culture medium of the synovial cells contains an inhibitor of collagenase as well as latent enzyme; t...

Efficacy of optimized in vitro predegeneration period on the cell count and purity of canine Schwann cell cultures

Objective(s): Predegeneration is a standard technique to obtain mitotically activated and enriched cultures of Schwann cells (SCs). This study, for the first time, evaluated the impact of various duration of predegeneration on cell yield and enrichment of SCs from dog peripheral nerve. Materials and Methods: Dog sural nerves were subjected to 5, 10, 15 day-long in vitro predegeneration. The tot...

Preparation and characterization of a soluble precipitating antigen from African horsesickness virus propagated in cell cultures

Identifying Parameters in Parabolic

In this paper, we investigate the nite element method for numerically identifying physical parameters in parabolic initial-boundary value problems. The identifying problem is rst formulated as a constrained minimization problem using the output least squares approach with the H 1-regularization or BV-regularization. Then a simple nite element method is used to approximate the constrained minimi...